Description
eGFP is an enhanced green fluorescent protein with maximum excitation/emission wavelengths of 488 nm and 509 nm, respectively. When transfected into cells, it can express strong green fluorescence, making it suitable as a control in studies of target gene transfection and expression in mammalian cells.
This product is synthesized using the T7 High Yield RNA Transcription kit (Novoprotein, Cat.No.: E131) and modified with the Cap 1 Capping System (Novoprotein, Cat.No.: M082) to achieve a mature mRNA structure with a 5' Cap 1 structure and a 3' poly(A) tail. By introducing N1-Me-Pseudo UTP to replace natural UTP, the immunogenicity of the mRNA in mammalian cells is effectively reduced, while enhancing its stability. It is an ideal choice for studying transfection and expression through various assay methods.
