Description

mCherry is a red fluorescent protein derived from mushroom coral, widely used in biotechnology as a red fluorescent dye for tracking applications, including molecular labeling and localization of cellular components. Compared to other red fluorescent proteins, mCherry exhibits superior fluorescence brightness, photostability, and lower cytotoxicity. mCherry mRNA (N1-Me-Pseudo UTP) is a reporter gene mRNA encoding the red fluorescent protein mCherry, with maximum excitation and emission wavelengths of 587 nm and 610 nm, respectively. This product is synthesized using the T7 High Yield RNA Transcription kit (Novoprotein, Cat.No.: E131) and modified with the Cap 1 Capping System (Novoprotein, Cat.No.: M082) to achieve a mature mRNA structure with a 5' Cap 1 structure and a 3' poly(A) tail (Figure 1). By introducing N1-Me-Pseudo UTP to replace natural UTP, the immunogenicity of the mRNA in mammalian cells is effectively reduced, while enhancing its stability. It is an ideal choice for studying transfection and expression through various assay methods.