Description
Taq DNA Polymerase is a thermostable recombinant DNA polymerase that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´ flap endonuclease activity. The enzyme lacks a 3´→5´exonuclease activity (no proofreading ability). Taq DNA Polymerase leaves an “A” overhang, which makes the enzyme ideal for TA cloning.
This product is obtained by expressing the Thermus aquaticus DNA Polymerase gene in E. coli and then purifying it several times. It is supplied with 5× Taq Buffer (with Mg2+), more ideal amplification results can be obtained.
