E127-03 - DNase I (Glycerol-Free)

DNase I is an endonuclease that can randomly degrade single stranded or double stranded DNA with equal efficiency to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA:DNA hybrids. The bioactivity of DNase I is highly dependent on Ca²⁺, Mg²⁺ or Mn²⁺. In the presence of Mg²⁺, DNase I cleaves each strand of dsDNA independently in a statistically random fashion. In the presence of Mn²⁺, the enzyme cleaves both DNA strands at approximately the same site, producing DNA fragments with blunt-ends or with overhang termini of only one or two nucleotides. DNase I (Glycerol-Free) is a glycerol free formulation of our regular DNase I and is well suited for automated high throughput testing, lyophilization or where accurate pipetting of small volumes are crucial. DNase I (Glycerol-Free) is available in different sizes and concentrations.