Description
NovoNGS ChiTag enzyme has the activity of breaking double-stranded DNA and binding to antibodies. Based on its function, we applied it to CUT&Tag for protein-DNA interaction research. CUT&Tag is a new method for studying protein-DNA interactions. Compared with traditional ChIP-seq, it has the following advantages: time-saving and high efficiency, small amount of cells required, low background signal, good repeatability, etc. It can even be used for single-cell sequencing. CUT&Tag is a powerful tool for studying the interaction between proteins and chromatin DNA in vivo, and is of great significance for research on gene regulation, epigenetics, etc.
Version 2.0 of ChiTag uses pAG-Tn5 to replace the original pA-Tn5, which improves efficiency and removes the restrictions of antibody selection
