Description
Luciferase refers to a group of enzymes in nature capable of producing bioluminescence, with the most well-known being firefly luciferase from Photinus pyralis, a North American firefly. Firefly luciferase exhibits luciferase activity without requiring post-translational modifications.
The sequence of this product originates from Photinus pyralis and has been enhanced through point mutations based on the wild-type sequence, significantly improving protein stability and its pH adaptability. Once inside the cell, this product expresses firefly luciferase, which catalyzes the oxidation of the substrate D-luciferin to oxyluciferin, producing bioluminescence at a wavelength of approximately 560 nm during the oxidation of D-luciferin. Firefly luciferase is widely used as a bioluminescent reporter gene, serving as a control in studies involving target gene translation efficiency, cell viability, and in vivo imaging in mammalian cells.
This product is synthesized using the T7 High Yield RNA Transcription kit (Novoprotein, Cat.No.: E131) and modified with the Cap 1 Capping System (Novoprotein, Cat.No.: M082) to achieve a mature mRNA structure with a 5' Cap 1 structure and a 3' poly(A) tail (Figure 1). Additionally, this product incorporates N1-Me-Pseudo UTP to replace natural UTP, effectively reducing the intrinsic immunogenicity of the mRNA in mammalian cells and enhancing its stability, making it an ideal choice for both in vitro and in vivo studies.
