Description
The Cas protein (Cas9), discovered in Streptococcus pyogenes, is one of the most widely used Cas proteins. Cas9 functions as a DNA nuclease within the CRISPR genome-editing system. Guided by specific sgRNA, Cas9 targets designated sites in the genome and cleaves double-stranded DNA (dsDNA), resulting in double-strand breaks (DSBs).
Cas9 mRNA encodes the Cas9 protein, which is equipped with nuclear localization signals (NLS) at both the N- and C-termini to facilitate nuclear entry, thereby enhancing DNA cleavage efficiency. Additionally, the C-terminal Flag epitope tag aids in the detection, isolation, and purification of Cas9 protein.
This product is a mature mRNA synthesized in vitro using T7 RNA Polymerase 2.0 (Novoprotein, Catalog No.: E122), featuring a 5' Cap 1 structure and a 3' poly(A) tail. By introducing N1-Me-Pseudo UTP to replace natural UTP, the immunogenicity of the mRNA in mammalian cells is effectively reduced, while enhancing its stability. It is an ideal choice for studying transfection and expression through various assay methods.
