Description
The Cas protein (Cas9), discovered in Streptococcus pyogenes, is one of the most widely used Cas proteins. Cas9 functions as a DNA nuclease within the CRISPR genome-editing system. Guided by specific sgRNA, Cas9 targets designated sites in the genome and cleaves double-stranded DNA (dsDNA), resulting in double-strand breaks (DSBs).
Cas9 mRNA encodes the Cas9 protein, which is equipped with nuclear localization signals (NLS) at both the N- and C-termini to facilitate nuclear entry, thereby enhancing DNA cleavage efficiency. Additionally, the C-terminal Flag epitope tag aids in the detection, isolation, and purification of Cas9 protein.
This product is a mature mRNA synthesized in vitro using T7 RNA Polymerase 2.0 (Novoprotein, Catalog No.: E122), featuring a 5' Cap 1 structure and a 3' poly(A) tail. It is an ideal choice for studying transfection and expression through various assay methods.
