Description
Luciferase refers to a group of enzymes in nature capable of producing bioluminescence, with the most well-known being firefly luciferase from Photinus pyralis, a North American firefly. Firefly luciferase exhibits luciferase activity without requiring post-translational modifications.
The sequence of this product originates from Photinus pyralis and has been enhanced through point mutations based on the wild-type sequence, significantly improving protein stability and its pH adaptability. Once inside the cell, this product expresses firefly luciferase, which catalyzes the oxidation of the substrate D-luciferin to oxyluciferin, producing bioluminescence at a wavelength of approximately 560 nm during the oxidation of D-luciferin. Firefly luciferase is widely used as a bioluminescent reporter gene, serving as a control in studies involving target gene translation efficiency, cell viability, and in vivo imaging in mammalian cells.
This product is a circular RNA synthesized in vitro using the T7 High Yield RNA Transcription Kit (Novoprotein, Cat.No.: E131) and a type I intron self-splicing mechanism. The unique covalently closed circular structure of the RNA prevents degradation by exonucleases, offering significantly higher biological stability compared to linear.
