Description
Cre (Cyclization Recombination Enzyme) is a 38 kDa DNA recombinase isolated from the P1 bacteriophage. Cre specifically recognizes loxP DNA sequences and mediates site-specific deletion of DNA sequences located between two loxP sites. The Cre-loxP system is primarily used for gene knockout and can also induce inversion or translocation of DNA sequences depending on the position and orientation of the loxP sites. This system has been widely applied to generate conditional gene expression/knockout/knockdown mouse models.
This product is mature mRNA synthesized in vitro using T7 RNA Polymerase 2.0 (Novoprotein, Catalog No.: E122), featuring a 5' Cap 1 structure and a 3' poly(A) tail. Additionally, by incorporating N1-Me-Pseudo UTP to replace natural UTP, it effectively reduces the intrinsic immunogenicity of mRNA in mammalian cells while enhancing its stability.
